0. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. TheseInstructions on the proper use of the SpectraMax M5 Plate Reader. 1. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 97 cAMP Detection AssayThe SpectraMax M5e reader offers the additional benefit of being certified . You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. These robust readers have been placed in labs from Antarctica to the. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. reader (FI-FLRH) SpectraMax Paradigm. SpectraMax Mini: 17. Ends from. Measured on the SpectraMax M5e™ reader, AcGFP (jellyfish), ZsGreen (reef coral), DsRed (reef coral) fluorescent proteins expressing cells can be assayed on a single plate, down to approximately 30 cells per well (384-well format), highlighting the increased light throughput efficiency and sensitivity of the MDS Analytical Technologies. measured using a microplate reader (SpectraMax M5e, Molecular Devices, San Jose, CA, USA) with excitation and emission at 494 and 520 nm, respectively, and calibrated against a standard curve for FITC. 303 unable to cal dark current. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. 5, Z’ = 0. Table of Contents. combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. 1. Microplate Readers. Gemini XPS. Two cynomolgus monkeys (1 male and 1 female) weighing 2–4 kg and 3–5 years old were purchased from Suzhou. This Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader is in good working order. 1. 40266 Email: drugdiscoverytech@lifetech. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. No special upgrade is required for HTRF ® readout, as it is a monochromator-based instrument. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. 00 USD. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. View the Molecular Devices website. NanoRacks LLC officials have chosen Molecular Devices’ SpectraMax M5e Multi-Mode Microplate Reader for use in NASA's International Space Station. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. In the M2, M5 and Plus. The CellTiter-Glo® 2. 97 cAMP Detection AssayMolecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. SpectraMax M5e: 118. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. 0. 1. Supplier: Molecular Devices. Homogeneous Time-Resolved Fluorescence (HTRF) . The REF button has no valid meaning for. For more sample throughput, the system can Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 5. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical-based and cell-Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader Chosen to be the First Microplate Reader in Space by NanoRacks, LLC for NASA’s International Space Station Sunnyvale, CA – May 17th, 2011 – Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research,Configure your microplate reader. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Fluorescence (FL). The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax M5e reconfigured to operate comfortably in the zero-gravity environment of the space station In May 2011, Molecular Devices’ SpectraMax ® M5e became the first microplate reader in space when NanoRacks added it to their line of commercial research hardware on the International Space Station, providing researchers the ability to. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Venue Address. #M5e) Methods Microplate reader compatibility test. 6 x 10-15. Add to cart. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Enzyme titration and time course, cofactor and substrate Km,andIC50 for known inhibitors are demonstrated. The NanoRacks Plate Reader-2 is a reconfigured SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, and has been configured to operate comfortably in the microgravity environment of the space station. 6 x 10-15. book Page 1 Wednesday, July 7, 2010 2:09 PM. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 8. The SpectraMax M5e possesses five preferred modes for multiple detection benefits in one platform: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Time-Resolved Fluorescence (TRF), Fluorescence. Other technologies such as imaging. Five preferred detection modes. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects andThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Lift up the lid over the chamber to insert or remove a cuvette. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes SpectraMax M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes Generation of NADPH was monitored by fluorescence (Ex340/Em460 nm) every minute for 15 minutes on a SpectraMax M5e plate reader (Molecular Devices at 25°C). . Plate chamber design of the SpectraMax category 1 microplate readers. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. SpectraMax ® ® M5e readers must be appropriately configured for HTRF ® readout by setting up the measurement conditions in the SoftMax Pro software. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. 0001 0. 5. FlexStation™ II. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. Call 516-939-0888 to check current. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Example: [Drawer]. The system had to pass vibration load, zero gravity, and temperature fluctuation testings. Features. A suspicious death, an upscale spiritual retreat, and a quartet of suspects with a motive for murder. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Introduction. S. M5e = 174nM SpectraMax M5e delta F % Paradigm = 18nM M5e = 19nM Figure 1. 1. 5. Fig 1. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Path Check Pathlength Measurement We would like to show you a description here but the site won’t allow us. #M5e) Methods Cell preparation. 00 USD. 6 x 10-15. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). SpectraMax® M5e. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. Molecular Devices SpectraMax M5e Microplate Reader. The dual monochromator optics allow. 2. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Products : SpectraMax M3 reader, SoftMax Pro Software, SpectraMax M5/M5e reader, SpectraMax L reader, SpectraMax M4 reader Is the Gaussia Luciferase (GLuc) Assay from New England Biolabs compatible with my plate reader? The information for the GLuc Assay Kit (Cat no. The KeySpan USA-19HS is often required with new Windows 7. 6 x 10-15. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. application or budget. About Conquer. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. HTRF assay performance on the SpectraMax M5e reader is demonstrated here with two different HTRF assay kits, cAMP dynamic 2 and IP-One. 6 M HCl for 24 h. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. FlexStation II. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. 3. SpectraMax Absorbance, Fluorescence, Luminescence, and Multi-Mode Readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 72 MB. Thermo Lab Systems Multiskan Ascent 354 $1,400. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. If not, our scientific teamwill help you choose the instrument that best suits your needs and budget. KEY FEATURES Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. SpectraMax Mini: 17. Molecular Devices. SoftMax Pro 软件模板设置之一标准曲线的绘制以及样品浓度的自动计算. The self-funded NanoRacksSpectraMax® Paradigm® Multi-Mode Detection Platform. Homogeneous Time-Resolved Fluorescence (HTRF) . SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The mirror set. 1. Bottom-reading is usually recommended for cell-based assay with adherent cells. 8 x 10-15. 1. The SpectraMax M2 and M2e are versatile and reliable. 1. SpectraMax Mini. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. AquaMax® 4000. SpectraMax M2. Meso Scale Discovery 1250 Sector Imager 2400 $ 2,500. In this product video, see how the SpectraMax M5 quickly reads samples. SpectraMax Mini: 17. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. OD 450 nm was measured by SpectraMax M5e. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. FlexStation™ III. book Page 1 Wednesday, July 7, 2010 2:09 PM. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode Multi-mode Microplate Readers. 0001 0. IMAP assays are based on binding of. SUNNYVALE, Calif. Choose from a three- (M3), four- (M4), orHome; Lighting; Work lights; User manual. It was difficult and time consuming to undertake quality, repetitive research. com A. 1 & 7. SpectraMax M3, M4, M5, and M5 e. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. View the Molecular Devices website. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Fluorescence polarization was measured using SpectraMax M5e plate reader (Molecular Devices): 485 nm excitation (9 nm bandwidth), 525 nm emission (15 nm bandwidth). Both instruments demonstrated good performance for the assay. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Paradigm: W = 21. G-protein coupled receptors signal through two major pathways, regulation of cAMP and increase in intracellular calcium levels mediated by IP3. The M5 has two PMTs. 485. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3 reader What is the best way to use fluorescence (FL) mode with cuvettes? 1. , contact Molecular. The Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader and computer with preload software (StakMax Microplate Handling System/racks not included). When the user applies a set temperature of 37°C, the top is heated to 37. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. SpectraMax M5/M5e/M4/M3/M2/M2e. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. The following document is intended to demonstrate setup of this instrument. SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Example: [Drawer]. FlexStation™ I. Read mode. Find regional support. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. No disponible por el momento. C. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. SpectraMax® L (software version 5. 5. In particular, these parameters should be entered as defined in the table below. . 9000-0756. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s experiment fromMolecular Devices SpectraMax™ M5e Microplate Reader w/ Warranty (Svc Contract Available) $34,000. Molecular Devices SpectraMax M5e Microplate Reader. It was difficult and time consuming to undertake quality, repetitive research. Introduction. (PRWEB) July 19, 2016 -- Molecular Devices, LLC, a leader in protein and cell biology technologies, today announced the launch of the SpectraMax. 1. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Page 8 FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Based on the SpectraMax M5e instrument platform, the FlexStation 3 instrument can address detection modalities including absorbance, fluorescence intensity, fluorescence polarization, time-resolved fluorescence, and luminescence. Specifications Figure A-3 Top view of a SpectraMax reader ® Common Fluorescence and Luminescence Wavelengths Values in the following tables are based on the literature. 41 nM were calculated using SoftMax Pro Software. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. SpectraMax M3, M4, M5, and M5 e. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. 2 x 10-14. reader. 1) SpectraMax Glo Steady Luc Reporter Assay (6. Ferguson, Ph. Gemini EM Gemini XPS SpectraMax M2 SpectraMax M2e SpectraMax M3 SpectraMax M4 SpectraMax M5 SpectraMax M5e SpectraMax Plus384 SpectraMax 340PC384 SpectraMax 190. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellNanoRacks, LLC is installing a new, reconfigured Molecular Devices’ SpectraMax M5e multi-mode microplate reader on the International Space Station to perform experiments in microgravity. StakMax Handling Stacker Base Unit. 2 x 10-14. The analysis capabilities of the software provide automated quantitation in an easy-to-read, user-customizable report format. SpectraMax L: 36. The following document is intended. Recommended Optics Wavelength (nm) Wavelength selection Excitation 332/9 Monochromator Emission 1 488/15. After excluding the background values, the cross‐reactivity of the. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. Both instruments demonstrated good performance for the assay. The assay was set up in a low-volume 384- well black plate using the pipetting. Instruments and Settings for Fluorescence Read-out (Other Fluorescent Assays) FLUOstar Omega, VANTAstar, CLARIOstar, PHERAstar, PolarStar Optima. 1. The mirror set. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. 316 grating motor driver faulty. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Table of Contents. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. spr SpectraMax Plus 384. AVALANCHE. SpectraMax M5e Reader only Certified to Cisbio Bioassays HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 0200-0729. Listing SKU: DIS-58825-molecular-devices-spectramax-m5e-microplate-readerIn May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader in space. 1)M5e = 174nM SpectraMax M5e delta F % Paradigm = 18nM M5e = 19nM Figure 1. The optical systemsApplication note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. S. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. 305 reference level saturation. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader with One Year Warranty. 5. 5. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 8. SpectraMax Glo and DuoLuc. 1. 5, Z’ = 0. ₹ 1,80,000 Get Latest Price. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The self-funded NanoRacks Plate Reader-2 features a wide range of high-performance multi. On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. Page 75. SpectraMax Paradigm. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. . SpectraMax Mini: 17. The following day, calcium content was quantified by the QuantiChrom™ Calcium Kit (BioAssay Systems) by reding absorbance at 612 nm using the Spectramax M5e reader (Molecular Devices). elegans, and Alcanivorax borkumensis. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter. 00We would like to show you a description here but the site won’t allow us. Sunnyvale, Calif. Oxygen consumption is one of the most informative and direct measures of mitochondrial function. After the firefly luciferase was measured, Dual-Glo Stop & Glo substrate solution (25 µL/well) was added offline to each well to quench the firefly luciferase reaction and provide substrate for the Renilla luciferase reaction. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. , May 17, 2011 /PRNewswire/ -- Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax® M5e Multi-Mode Microplate Reader for use in NASA's International Space Station; the. Reads various kinds of 96-wells plates, and is equipped with shaking function. 4. HTRF cAMP calibration curve HTRF cAMP calibration curve measured on the SpectraMax i3 (red circles), SpectraMax. Molecular Devices multi-mode microplate reader, mod SpectraMax M5e, s/n MVE05702 [East Research Lab] Auction Details Bio-Based Mushroom Leather Manufacturing Facility. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. We would like to show you a description here but the site won’t allow us. book Page 1 Wednesday, July 7, 2010 2:09 PM Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. The plate was agitated briefly, incubated 15 minutes at room temperature in the dark and read on M5e. M3-M5e_manual. book Page 1 Wednesday, July 7, 2010 2:09 PM. Cell monolayers were decalcified with 0. Meso Scale Discovery 1200 Sector Imager 2400. The corrected absorbance is within 5% of the. 2222 Fifth Street. Following the user manual for installation, maintenance, assembly or troubleshoot is an crucial prerequisite for safety in addition to protect consumers from any liability from the manufacturer of the product for viable defects in case of an insurance claim. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 5 mP standard deviation at 1 nM. Also refer to this Multimode Reader Comparison Chart. SpectraMax M5e: 118. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Cellular Imaging Systems: Molecular Devices offers various imaging solutions for your research needs. 00. 3. FlexStation II 384. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. The measurements were performed in triplicate. Delivers single mode reader performance in one multimode reader package. 306 plate air cal fail,low light. SpectraMax M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 00. Share Tweet Share Pin. Description 1. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. 1. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. GE AKTA™ Crossflow. Specifications Figure A-3 Top view of a SpectraMax reader ® Common Fluorescence and Luminescence Wavelengths Values in the following tables are based on the literature. 0200-7027. Jul 13, 2023 10am PT Date Format. Commenting on the launch, Christopher Silva, Director of Life Sciences Marketing at Molecular Devices, stated, "The HTRF(R) assay platform has given researchers an alternative set of kinase and GPCR assays, in. 8 x 10-15. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. 3. 6 x 10-15. Examples: “Press carefully to engage,” “ Do not. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator SpectraMax M5e Reader only Certified to Cisbio Bioassays’ HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. M5e: W = 15. Z1. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 01) + 0. SpectraMax M3, M4, M5, and M5e Multi-Mode Microplate Readers 0112-0115 F 9the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax® M5e Multi-Mode Microplate Reader (Molecular Devices) Assay setup and detection. 1. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. In this. KEY FEATURESMolecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Real-time fluorescence signals were recorded using CFX96 thermocycler (Bio-Rad). HTRF测定在SpectraMax M5e读板机上的 表现通过使用两种不同的HTRF检测试剂 展示。G-蛋白偶联受体信号转导通过检测 两种主要通路，cAMP的调控和IP3介导的 胞内钙离子水平增加。利用cAMP dynamic 2试剂中Eu的穴状化合物标记的 单克隆抗-cAMP抗体和d2-标记的cAMP进 行检测。The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The absorbance of the wells containing only the primary antibodies but no antigen was used as the background value. WARNING! The instrument weighs approximately 36 pounds. After washing, streptavidin-HRP conjugate (Jackson ImmunoResearch Laboratories; 016-030-084) was added for 1 hour as per the manufacturer's instructions, plates washed and incubated with TMB (KPL), and absorbance at 450 nm was measured on a SpectraMax M5e with SoftMax Pro Software (Molecular Devices). Conclusions. If you love a cozy, comedic mystery, you'll love this 'whodunit' adventure. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart . Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Results summary for cAMP HiRange standard curve Figure 3. Using. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. . Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. Supplier: Molecular Devices. 8.